Abstract: This article takes the newly-built Haihe road in Xinxiang High-tech Zone passing through mengjiangnvhe bridge of Beijing Guangzhou high-speed railway as an example, the influence of vehicles on bridge piers and foundation of high-speed railway after construction and operation of Haihe road under Beijing Guangzhou high-speed railway is studied and analyzed. The results show that: during the construction and operation of Haihe road project Undercrossing Beijing Guangzhou high-speed railway protection project, the displacement and settlement of No. 196-201 pier of mengjiangnuhe super large bridge on Beijing Guangzhou high-speed railway and axial force of pile foundation of high-speed railway are affected. The maximum vertical, longitudinal and transverse displacements of no.196-201 piers of mengjiangnuhe super large bridge are -0.502 mm, 0.465 mm and 0.139 mm respectively, which meet the requirements of 2 mm limit in the regulations.